Identification of higenamine and its metabolites in rat by gas chromatography/mass spectrometry

Autor:	Jae-Chun Ryu, Yun-Seon Song, Hye Sook Yun-Choi, Myungsoo Kim, Jung-Hyuck Cho
Rok vydání:	1993
Předmět:	Higenamine Detection limit Chromatography biology Metabolite Organic Chemistry Ranunculaceae Mass spectrometry Mass chromatogram biology.organism_classification chemistry.chemical_compound chemistry Drug Discovery Molecular Medicine Gas chromatography–mass spectrometry Coclaurine
Zdroj:	Archives of Pharmacal Research. 16:213-218
ISSN:	1976-3786 0253-6269
Popis:	(±)-Higenamine is known as a cardiotonic principle of aconite root (root ofAconitum spp., Ranunculaceae). A simple and sensitive detection method for higenamine was developed by using gas chromatography-mass spectrometry (GC/MS). The recovery of higenamine after extraction and concentration with XAD-2 resin column was around 95% from rat biological fluids such as bile, plasma and urine. The limits of detection of higenamine in these biological fluids were approximately 0.1 ng/ml each. It has well been suggested that tetrahydroisoquinolines possessing catechol moiety such as higenamine should be subjected to the catechol-O-methyl transferase (COMT) activityin vivo. We detected two major peaks of presumed metabolites of higenamine in the total ion chromatogram obtained from the rat urine sample after the oral administration of (±)-higenamine. The scan mass spectrum of one of the metabolites coincided with those obtained from coclaurine (C6-O-methyl higenamine) and those of the other metabolite are suggestive of isococlaurine (C7-O-methyl higenamine).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4aba7f3c67e4b6365d5a3e04c1383a3f https://doi.org/10.1007/bf02974485 Zobrazit plný text záznamu Full text from SpringerLink