| Autor: |
Amany Moussa Husseiny, Noha Nagi Salah El-Deen, Randa Mohamed Kamal Abd-EL Raouf, Safaa S. Imam, Maha Muhammad Fathy |
| Rok vydání: |
2016 |
| Předmět: |
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| Zdroj: |
The Egyptian Journal of Medical Microbiology. 25:17-24 |
| ISSN: |
1110-2179 |
| Popis: |
Background: Early neonatal acquisition of Ureaplasma urealyticum (U. urealyticum) and Ureaplasma parvum (U. parvum), in the 1 st 24 hours after birth and during passage of an infected maternal birth canal, have been linked to the development of respiratory diseases in premature newborns as a result of respiratory tract colonization. However, the role of these pathogens in the development of respiratory distress syndrome (RDS) still needs more clarification. Objectives: In this study we aimed to search for U. urealyticum and U. parvum in tracheal aspirates of premature infants with RDS by species-specific PCR assays and to evaluate the short term outcome of preterm infants with positive results. Methodology: Tracheal aspirate fluid samples from 35 preterm neonates with RDS with a mean gestational age of (32.76±2.48) weeks were collected within the first 24 hours after birth and analyzed for detection of U. urealyticum and U. parvum by species-specific PCR assays targeting urease gene subunits and adjoining spacer regions. Results: Ureaplasma spp. was detected in the tracheal aspirate of 6 (17%) preterm infants with RDS. Among these 6 positive cases; 5 (83%) were positive for U. parvum while only 1 case (17%) was positive for U. urealyticum. The preterm infants with positive results for Ureaplasma spp. had statistically significant higher degree of RD depending on the chest X ray grading results and longer durations of ventilation and hospitalization. Conclusions: Limited by the relatively small sample size, this work can be considered as a pilot study indicating a possible association between the severity of RDS in preterm infants and respiratory tract (RT) colonization with Ureaplasma spp. particularly U.parvum. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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