Identification of circulating miRNA involved in meat yield of Korean cattle
| Autor: | Surim Lee, Myunggi Baik, S. J. Park, Jong-Youl Ko, Jae-kyoung Cheong, Jin-Jong Bong |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Circulating mirnas Microarray business.industry Myogenesis Cell Biology General Medicine Beef cattle Biology Biotechnology law.invention Andrology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine law 030220 oncology & carcinogenesis microRNA Epigenetics business Gene Polymerase chain reaction |
| Zdroj: | Cell Biology International. 41:761-768 |
| ISSN: | 1065-6995 |
| DOI: | 10.1002/cbin.10776 |
| Popis: | Cattle plays an important role in providing essential nutrients through meat production. Thus, we focused on epigenetic factors associated with meat yield. To investigate circulating miRNAs that are involved with meat yield and connect biofluids and longissimus dorsi (LD) muscle in Korean cattle, we performed analyses of the carcass characteristics, miRNA array, qPCR, and bioinformatics. Carcass characteristics relative to the yield grade (YG) showed that the yield index and rib eye area were the highest, whereas the backfat thickness was the lowest for YG A (equal to high YG) cattle among the three YGs. miRNA array sorted the circulating miRNAs that connect biofluids and LD muscle. miRNA qPCR showed that miR-15a (r = 0.84), miR-26b (r = 0.91), and miR-29c (r = 0.92) had positive relationships with biofluids and LD muscle. In YG A cattle, miR-26b was considered to be a circulating miRNA connecting biofluids and LD muscle because the target genes of miR-26b were more involved with myogenesis. Then, miR-26b-targeted genes, DIAPH3 and YOD1, were downregulated in YG A cattle. Our results suggest that miR-15a, miR-26b, and miR-29c are upregulated in biofluids and LD muscle, whereas DIAPH3 and YOD1 are downregulated in the LD muscle of finishing cattle steers. |
| Databáze: | OpenAIRE |
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