Rare triterpene glycoside of ginseng (ginsenoside malonyl-Rg1) detected in plant cell suspension culture of Panax japonicus var. repens
| Autor: | E. S. Glagoleva, Alexander Nosov, M. V. Titova, D. V. Kochkin, B. A. Galishev |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine chemistry.chemical_classification Chromatography biology Electrospray ionization fungi food and beverages Glycoside Plant physiology Plant Science Malonic acid biology.organism_classification 01 natural sciences Repens 03 medical and health sciences chemistry.chemical_compound Ginseng 030104 developmental biology chemistry Triterpene Ginsenoside 010606 plant biology & botany |
| Zdroj: | Russian Journal of Plant Physiology. 64:649-656 |
| ISSN: | 1608-3407 1021-4437 |
| DOI: | 10.1134/s102144371705003x |
| Popis: | This paper reports for the first time about the detection and identification of ginsenoside malonyl-Rg1 (the rare 20(S)-protopanaxatriol-type ginsenoside) in the biomass of plant cell suspension culture of Japanese ginseng (Panax japonicus C.A. Mey. var. repens). Ginsenosides were analyzed by means of high-performance liquid chromatography/electrospray ionization mass spectrometry (HPLC-ESI-MS) in positive-ion mode. Malonyl-Rg1 was identified as a result of interpretation of MS spectra obtained upon fragmentation of protonated molecular ion ([M + H]+) of this compound in an ionization source. Chromatographic analysis and MS spectra showed that the cells of P. japonicus var. repens cultivated in vitro contain several isomers of malonyl-Rg1. Thus, we ascertained for the first time that, in addition to malonyl ginsenosides of 20(S)-protopanaxadiol group, the plant cell culture of ginseng P. japonicus var. repens can accumulate glycosides of 20(S)-protopanaxatriol group acylated with a malonic acid residue. The obtained results showed that, in the cells of ginseng cultivated in vitro for a long time (for 10 years and more), the assortment of secondary metabolites (ginsenosides) may be as wide as in intact plants. |
| Databáze: | OpenAIRE |
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