| Popis: |
1. A study has been made of a double-fronting effect appearing in one-step development chromatography on anion exchangers. 2. It has been shown that the phenomenon is due to interaction with atmospheric carbon dioxide, and that it can be avoided by working with a carbonate-free system. 3. Possible mechanisms for the double-fronting have been discussed. 4. e-DNP-lysine has been split up into two zones by one-step development on diethylaminoethyl cellulose (DEAE cellulose), thus showing the possibilities of artifacts caused by the double-fronting. |
