ACTIVITY OF SUPEROXIDE DISMUTASE, GLUTATHIONE PEROXIDASE, GLUCOSE-6-PHOSPHATE DEHYDROGENASE IN ERYTHROCYTES OF UNTRAINED RATS IN 'TO-MUSCULAR-FAILURE' SWIMMING TEST
| Autor: | E.R. Boyko, N A Vakhnina, V. D. Shadrina |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification medicine.medical_specialty biology Chemistry Glutathione peroxidase 030204 cardiovascular system & hematology Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Endocrinology Internal medicine medicine biology.protein Glucose-6-phosphate dehydrogenase |
| Zdroj: | Ulyanovsk Medico-biological Journal. :133-141 |
| ISSN: | 2227-1848 |
| DOI: | 10.34014/2227-1848-2020-4-133-141 |
| Popis: | For an untrained organism, physical activity is physiologically stressful. The stress is accompanied by an increase in reactive oxygen intermediates. Their level in the cell is regulated by antioxidant enzymes. The aim of the paper to study the activity of superoxide dismutase (SOD), glutathione peroxidase (GP) and glucose-6-phosphate dehydrogenase (G-6-FDH) in erythrocytes under single physical load (FL) of different intensity. Materials and Methods. The authors tested male Wistar rats aged 3 months. The animals were divided into seven groups: vivarium control (VC); rats who were swimming for 60 minutes without added weight (moderate intensity load (MIL)); four groups participating in load-testing: low-intensity FL (LI) – swimming with a load of 2 % of body weight; high-intensity FL (HI) - swimming with a load of 8 %, 10 % and 15 % of body weight (HI8, HI10, HI15). Animals of the seventh group (OS) were in the shallows for 60 min without the opportunity to swim in order to induce oxidative stress without physical load. Results. In all groups, the authors observed a significant decrease in SOD activity and a significant increase in GP and G-6-FDH activity relative to VC. The greatest decrease in SOD activity was in the OS and MIL groups (by 36 % and 33.5 %, p |
| Databáze: | OpenAIRE |
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