Analysis ofβ-Lactam Antibiotics Using a Microbial Receptor Protein-based Biosensor Assay

Autor:	Peter Bjurling, Åse Sternesjö, Jacques Degelaen, Eva Gustavsson
Rok vydání:	2002
Předmět:	Detection limit Maximum Residue Limit Immunology Cephalosporin C Penicillin chemistry.chemical_compound chemistry Biochemistry polycyclic compounds Lactam medicine Receptor Agronomy and Crop Science Biosensor Food Science medicine.drug Conjugate
Zdroj:	Food and Agricultural Immunology. 14:121-131
ISSN:	1465-3443 0954-0105
DOI:	10.1080/09540100220145142
Popis:	An optical biosensor assay for detection of β-lactam antibiotics in milk based on a microbial receptor protein was developed. The assay uses a general sensor surface previously described with a small organic molecule (H1) immobilized. A conjugate between a β-lactam (cephalosporin C) and a monoclonal H1 antibody is injected across the sensor surface before injection of the sample mixed with receptor protein. Receptor inhibited by β-lactam residues in the milk sample will not bind to the sensor surface and the reduction in response is inversely related to the β-lactam concentration of the sample. The detection limit for a number of commonly used β-lactams was below or near the respective maximum residue limit and the relative standard deviation (CV) for penicillin G in milk was 6-12% in the interval 2.0-12.5 μ g kg-1. For application in the field further optimization is needed to solve problems related to non-specific binding to the sensor surface.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4a6e0f446f41213aca7b31ed94895126 https://doi.org/10.1080/09540100220145142 Zobrazit plný text záznamu Plný text ve formátu PDF