Screening DNA probes using the hydrophobic grid-membrane filter

Autor:	A.N. Sharpe, Edmund S. Idziak, P.I. Peterkin
Rok vydání:	1989
Předmět:	Hybridization probe Membrane filter Biology medicine.disease medicine.disease_cause biology.organism_classification Microbiology Molecular biology Hemolysis Plasmid dna Listeria monocytogenes medicine Listeria Food microbiology Genomic library Food Science
Zdroj:	Food Microbiology. 6:281-284
ISSN:	0740-0020
DOI:	10.1016/s0740-0020(89)80009-7
Popis:	Seven clones showing β -hemolysis, from a Listeria monocytogenes genomic library, were assessed by a hydrophobic grid-membrane filter (HGMF) screening technique for their specificity as DNA probes. Plasmid DNA from each of the clones was screened by colony hybridization against replicates of a library of 100 organisms, consisting of 70 L. monocytogenes strains, ten other Listeria spp., and 20 organisms of other genera, arrayed on an HGMF. Clones showing potential for use in analytical food microbiology were identified by this means.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4a697a0a0e38e98b9c4f60604cc6fe45 https://doi.org/10.1016/s0740-0020(89)80009-7 Zobrazit plný text záznamu