Simultaneous Detection and Direct Identification of Phytoplasmas in the Aster Yellows (16SrI), Clover Proliferation (16SrVI) and Stolbur (16SrXII) Groups Using a Multiplex Nested PCR Assay in Potato Plants
| Autor: | Majid Talebi, J. Gholami, Masoud Bahar |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
food and beverages 04 agricultural and veterinary sciences Ribosomal RNA Biology biology.organism_classification 01 natural sciences Virology Aster yellows Phytoplasma Disease management (agriculture) Multiplex polymerase chain reaction 040103 agronomy & agriculture Candidatus 0401 agriculture forestry and fisheries Multiplex Agronomy and Crop Science Nested polymerase chain reaction 010606 plant biology & botany Food Science |
| Zdroj: | Potato Research. 63:403-415 |
| ISSN: | 1871-4528 0014-3065 |
| DOI: | 10.1007/s11540-019-09447-8 |
| Popis: | Phytoplasmas in the aster yellows (16SrI), clover proliferation (16SrVI) and stolbur (16SrXII) groups have a wide host range with common hosts. In the current study, specific primers based on the 16-23S rRNA spacer region were designed and optimised for the detection and identification of phytoplasmas in these groups in a multiplex nested PCR assay. Fifty-two phytoplasma symptomatic potato plants were tested, and clover proliferation (16SrVI) and stolbur (16SrXII) phytoplasmas detected coinfecting 16 potato plants with single infection of these phytoplasmas in 5 and 21 samples, respectively. ‘Candidatus P. asteris’ (16SrI) was not detected. The specificity of the primers was proven in both single and multiplex PCR assays. Detection of multiple infections can be useful in assisting epidemiological studies and in deciding appropriate disease management strategies. |
| Databáze: | OpenAIRE |
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