Differential Control of Immunoreactive α-Inhibin and Progesterone Production by Marmoset Luteal Cells in Vitro: Evidence for a Paracrine Action of α-Inhibin on Basal and Gonadotropin-Stimulated Progesterone Production1
| Autor: | G. E. Webley, P. G. Knight, P. L. Marsden |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
endocrine system
medicine.medical_specialty endocrine system diseases medicine.drug_class medicine.medical_treatment Prostaglandin Ovary Biology Basal (phylogenetics) chemistry.chemical_compound Internal medicine biology.animal medicine reproductive and urinary physiology Marmoset Cell Biology General Medicine Progesterone secretion medicine.anatomical_structure Endocrinology Reproductive Medicine chemistry Gonadotropin Corpus luteum hormones hormone substitutes and hormone antagonists Prostaglandin E |
| Zdroj: | Biology of Reproduction. 50:1394-1402 |
| ISSN: | 1529-7268 0006-3363 |
| Popis: | There is an increase in plasma concentrations of immunoreactive (ir) inhibin unaccompanied by a rise in plasma progesterone during early pregnancy in the marmoset monkey. We investigated the potential involvement of hCG and prostaglandin E 2 (PGE2 ) in stimulating a selective increase in inhibin concentrations by measuring the production of ir-a-inhibin and progesterone by dispersed luteal cells cultured under serum-free conditions. After one day, hCG had no effect on progesterone production by the cells but stimulated a significant increase (p < 0.05) in a-inhibin production. PGE 2 significantly increased progesterone production (p < 0.001) but inhibited the production of a-inhibin (p < 0.001). After three days of culture, output of a-inhibin fell to low levels and no significant effect of hCG or PGE2 was detected. Progesterone also fell with time in culture, but hCG maintained production resulting in a significant increase above control levels (p < 0.001). The addition of low density lipoproteins (LDL) to the culture medium increased progesterone production (p < 0.001) while decreasing a-inhibin production (p < 0.01). Immunoneutralization of endogenous a-inhibin resulted in a significant decrease in both basal (p < 0.05) and gonadotropin-stimulated (p < 0.05) progesterone concentrations. These results provide further evidence for differential control of progesterone and at-inhibin production by marmoset luteal cells and show that hCG can selectively stimulate a-inhibin production. In addition, oa-inhibin may have a local paracrine action in the marmoset CL, enhancing both basal and gonadotropinstimulated progesterone secretion. |
| Databáze: | OpenAIRE |
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