Biological and chemical insight into Gaultheria procumbens fruits: a rich source of anti-inflammatory and antioxidant salicylate glycosides and procyanidins for food and functional application
| Autor: | Jarosław Rojek, Piotr Michel, Łukasz Poraj, Monika A. Olszewska, Sebastian Granica, Karolina Rosińska |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Antioxidant 010405 organic chemistry medicine.drug_class medicine.medical_treatment Glycoside General Medicine medicine.disease_cause 01 natural sciences In vitro Anti-inflammatory 0104 chemical sciences 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Enzyme chemistry Hyaluronidase medicine Food science Quercetin Oxidative stress Food Science medicine.drug |
| Zdroj: | Food & Function. 11:7532-7544 |
| ISSN: | 2042-650X 2042-6496 |
| DOI: | 10.1039/d0fo01750g |
| Popis: | The fruits of Gaultheria procumbens are traditionally used for culinary and healing purposes as anti-inflammatory agents. In the present work, the active components of the fruits were identified (UHPLC-PDA-ESI-MS3, preparative HPLC isolation, and NMR structural studies), and their biological capacity was evaluated in vitro in cell-based and non-cellular models. The fruits were revealed to be the richest known dietary source of salicylates (38.5 mg per g fruit dw). They are also rich in procyanidins (28.5 mg per g fruit dw). Among five tested solvents, acetone was the most efficient in concentrating the phenolic matrix (39 identified compounds; 191.3 mg g−1, 121.7 mg g−1, and 50.9 mg g−1 dry extract for total phenolics, salicylates, and procyanidins, respectively). In comparison to positive controls (dexamethasone, indomethacin, and quercetin), the extract (AE) and pure salicylates exhibited strong inhibitory activity towards pro-inflammatory enzymes (cyclooxygenase-2 and hyaluronidase). The analytes were found to be non-cytotoxic (flow cytometry) towards human neutrophils ex vivo. Moreover, they significantly, in a dose-dependent manner, downregulated the release of ROS, TNF-α, IL-1β, and elastase-2 and slightly inhibited the secretion of IL-8 and metalloproteinase-9 in the cells. The observed effects might support the usage of G. procumbens fruits as functional components of an anti-inflammatory diet and indicate the potential of AE for use in adjuvant treatment of inflammatory disorders cross-linked with oxidative stress and associated with the excessive production of TNF-α, IL-1β, and elastase-2. |
| Databáze: | OpenAIRE |
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