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Low productivity, high cost, and disbalance between swelling behavior and mechanical properties have limited extensive applications of bacterial cellulose pellicle (BCP). To remove the drawbacks, two-step fermentation and in-situ modification of chitosan oligosaccharide (COS) were jointly used to improve the yield and mechanical properties of BCP in this work. The results indicated that based on the optimization of the culture components, a higher yield of 5.5 g/L, which is 1.9-fold of the control produced under static fermentation, was achieved at the agitation speed of 220 rpm for 3 h (total incubation time 10 d), revealing that high agitation speed in the early incubation can promote BCP production and shorten the production time. In-situ addition of COS can induce the self-assembly of bacterial nanofibers to form a compact network of BCP through intermolecular hydrogen bond instead of intramolecular hydrogen bond, increasing the yield and mechanical properties. The yield increased to 10.8 g/L, approximately 2.0-fold that of the control. The Young’s modulus, toughness, yield strength, and resilience of BCP were increased by 5.7, 2.9, 3.1, and 4.0 folds, respectively. It is convinced that COS modification well improved BCP production and balanced the swelling behavior and mechanical properties. Consequently, this work removed the drawbacks of BCP production to a certain degree, which hopefully contributes to an extensive application of BCP. |
