A Modified Counting Method for Very Small Number of Residual White Blood Cells in Platelet Concentrates by Flow Cytometory

Autor:	Taiko Seno, Noriko Okuni, Hideo Yamaguchi, Hirotoshi Shibata, Yasuto Okubo, Mitsunobu Tanaka, Yoshio Kobayashi
Rok vydání:	1997
Předmět:	chemistry.chemical_compound chemistry business.industry Small number Platelet Propidium iodide Biology Residual Nuclear medicine business Staining Biomedical engineering
Zdroj:	Journal of the Japan Society of Blood Transfusion. 43:13-20
ISSN:	1883-8383 0546-1448
Popis:	A modified flow cytometric (FCM) method for counting very small numbers of residual white blood cells (WBCs) in platelet concentrates (PCs) was developed. Non-labeled chicken red blood cells (cRBCs) were added to samples of PCs as an internal control. After incubation with propidium iodide (PI) staining agent, residual WBCs and control cRBCs were counted using a Cytoron flow cytometer (Ortho-Clinical Diagnostics K. K. Tokyo, Japan). Average time needed to count one sample was 40 seconds. The range of cell numbers able to counted by this method was 1.6×102-105 cells per ml with good reproducibility.The advantages of this technique are that its rapid and accurate, requires only 0.2ml of PCs and is easy to perform. It therefore represents a useful method for quality control of residual WBCs in platelet concentrates.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::49e675edefd962dd63272bcd3aba9f24 https://doi.org/10.3925/jjtc1958.43.13 Zobrazit plný text záznamu