Standardizing for microsatellite length in comparisons of genetic diversity
| Autor: | Marie-France Deguilloux, Rémy J. Petit, Giovanni G. Vendramin, Pauline Garnier-Géré, Joëlle Chat, Delphine Grivet |
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| Rok vydání: | 2005 |
| Předmět: |
0106 biological sciences
Genetics 0303 health sciences Mutation rate Genetic diversity Biology 010603 evolutionary biology 01 natural sciences 03 medical and health sciences Chloroplast DNA Genetic variation Microsatellite Species richness Allele Allele frequency Ecology Evolution Behavior and Systematics 030304 developmental biology |
| Zdroj: | Molecular Ecology. 14:885-890 |
| ISSN: | 0962-1083 |
| DOI: | 10.1111/j.1365-294x.2005.02446.x |
| Popis: | Mutation rates at microsatellites tend to increase with the number of repeats of the motif, leading to higher levels of polymorphism at long microsatellites. To standardize levels of diversity when microsatellites differ in size, we investigate the relationship between tract length and variation and provide a formula to adjust allelic richness to a fixed mean number of repeats in the specific case of chloroplast microsatellites. A comparison between 39 loci from eight species of conifers (where chloroplast DNA is paternally inherited) and 64 loci from 12 species of angiosperms (where chloroplast DNA is generally predominantly maternally inherited) indicates that the greater allelic richness found in conifers remains significant after controlling for number of repeats. The approach stresses the advantage of reporting variation in number of repeats instead of relative fragment sizes. |
| Databáze: | OpenAIRE |
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