Iron supply to tobacco plants through foliar application of iron citrate and ferric dimerum acid

Autor: Georg Ebert, Victoria Fernández, Günther Winkelmann
Rok vydání: 2004
Předmět:
Zdroj: Physiologia Plantarum. 122:380-385
ISSN: 1399-3054
0031-9317
DOI: 10.1111/j.1399-3054.2004.00405.x
Popis: Experiments to assess the re-greening and the distribution of leaf-applied iron (Fe) within the aerial organs were developed with tobacco (Nicotiana tabacum L.) plants. Fe (III)-citrate and Fe-dimerum acid were applied to a part of the leaf and plant re-greening was monitored for 6 weeks after treatment. Fluid Fe increments associated with foliar Fe application were measured within 3 days after application. Fe distribution from the site of application within the leaf and the untreated above-ground plant parts was evaluated. Leaf fluid Fe concentration was determined by a novel procedure based on Fe chelation by desferrioxamine E and high performance liquid chromatography separation on a reversed-phase column. The ferrioxamine E method enabled accurate determination of small amounts of Fe present in leaf fluid. Foliar Fe-dimerum acid and Fe (III)-citrate treatment to chlorotic tobacco plants induced new growth re-greening. It was concluded that the applied Fe-containing compounds penetrated the leaf, were distributed within the plant and that Fe supplied as both complexes could be utilized by the plant cell.
Databáze: OpenAIRE