Comparison of ELISA and HPLC techniques for the analysis of carbendazim and thiabendazole residues in fruit and vegetables

Autor:	Shaun N. White, Stephen A. Thorpe, Katrina A. Mountfort, Stewart L. Reynolds
Rok vydání:	1994
Předmět:	Hplc analysis Chromatography medicine.diagnostic_test Chemistry Carbendazim Immunology High-performance liquid chromatography chemistry.chemical_compound Immunoassay medicine Maceration (wine) Elisa method Agronomy and Crop Science Food Science
Zdroj:	Food and Agricultural Immunology. 6:17-22
ISSN:	1465-3443 0954-0105
DOI:	10.1080/09540109409354808
Popis:	An enzyme‐linked immunosorbent assay (ELISA) was compared with a high‐pressure liquid chromatography (HPLC) procedure for the analysis of carbendazim and thiabendazole in fruit and vegetables. The method was found to be rapid, simple and repeatable; the main disadvantages being that the ELISA used could not distinguish between carbendazim and thiabendazole and that there were matrix effects from solvents and sample extracts. Consequently, a simple screening procedure was devised for carbendazim in fruit and vegetables. Samples were extracted by maceration with methanol and analyzed using immunoassay kits. Recoveries from 100 to 123% were achieved. The screening procedure was applied to samples of fruit and vegetables over a period of 12 months and compared with conventional HPLC methodology. The correlation coefficient between the two techniques was 0.91 and the ELISA method represented a saving of 54% in time and used only 12% of the solvent used for HPLC analysis.
Databáze:	OpenAIRE
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