Determination of Telomere Length by the Quantitative Fluorescence in Situ Hybridization (Q-FISH) Method

Autor:	Naotaka Izumiyama-Shimomura, Masanori Terai, Junko Aida, Shinsuke Aida, Naoshi Ishikawa, Tomio Arai, Kaiyo Takubo, Yoko Matsuda, Kenichi Nakamura
Rok vydání:	2014
Předmět:	Psychiatry and Mental health chemistry.chemical_compound chemistry Eukaryotic chromosome fine structure Chromosome instability Centromere Chromosome Q-FISH Karyotype Biology Molecular biology DNA Telomere
Zdroj:	American Journal of Analytical Chemistry. :775-783
ISSN:	2156-8278 2156-8251
DOI:	10.4236/ajac.2014.512086
Popis:	Telomeres are nucleoprotein complexes located at the ends of eukaryotic chromosomes and are shorten with aging or various causes. Shortened telomere plays an important role for chromosomal instability in carcinogenesis, or a number of diseases in relation to aging. FISH using peptide-nucleic acid (PNA) probe is suitable for analyzing telomeres, because telomere is a part of DNA molecule and localized in chromosomal end in loop shape structure (T-loop). We introduce our telomere analysis for tissue sections and cultured cells in this paper. On the tissue sections, we analyze telomere length with telomere intensity to centromere intensity ratio (TCR), centromere as an inner control. In the cultured cells, we analyze telomere lengths on each chromosome with karyotyping. Those Q-FISH methods by PNA probe are an accurate and reliable tool for research on telomere lengths in various conditions in biology.
Databáze:	OpenAIRE
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