Field‐evolved resistance to chlorpyrifos by Spodop tera frugiperda (Lepidoptera: Noctuidae): Inheritance mode, cross‐resistance patterns, and synergism

Autor: Oderlei Bernardi, Cínthia G Garlet, Ramon B Palharini, Daniela N. Godoy, Juliano Ricardo Farias, Rafaella P Moreira, Patricia da S Gubiani
Rok vydání: 2021
Předmět:
Zdroj: Pest Management Science. 77:5367-5374
ISSN: 1526-4998
1526-498X
DOI: 10.1002/ps.6576
Popis: Background Fall armyworm (FAW), Spodoptera frugiperda (Smith), is an economically important pest worldwide. In this study, we selected a genotype of FAW resistant to chlorpyrifos from a field-collected population, characterized the genetic basis of resistance, evaluated cross-resistance and mechanisms of resistance using synergists. Results The LD50 values of chlorpyrifos for the resistant (Clorp-R) and susceptible (Sus) FAW genotypes were 24.26 and 0.023 μg larva-1 , respectively, representing a resistance ratio >1,050-fold. The LD50 values of chlorpyrifos against heterozygotes were 3.34 and 4.00 μg larva-1 , suggesting that resistance is autosomally inherited. The chlorpyrifos resistance in FAW was influenced by few genes, with the minimum numbers of segregations being 1.74 and 1.88. On chlorpyrifos-sprayed plants and leaves, Clorp-R and heterozygotes genotypes showed >95% and >52% survival, respectively, whereas the Sus genotype had no survival, indicating that the resistance is incompletely dominant at the field rate of chlorpyrifos. The Clorp-R genotype presented some cross-resistance to acephate, but low cross-resistance to thiodicarb, methomyl, chlorfenapyr, flubendiamide, methoxyfenozide, spinetoram, and teflubenzuron. The synergists piperonyl butoxide, diethyl maleate and S,S,S-tributyl phosphorotrithiotate did not have relevant effects on the Clorp-R genotype, suggesting a minor role for metabolic resistance. Conclusions The inheritance of chlorpyrifos resistance in FAW was characterized as autosomal, incompletely dominant and polygenic, with metabolic resistance playing a small role in the detoxification of chlorpyrifos. Low cross-resistance between chlorpyrifos and other Mode of Action (MoA) insecticides occurs in FAW, highlighting the importance of considering the rotation of MoA as a strategy to delay resistance. This article is protected by copyright. All rights reserved.
Databáze: OpenAIRE
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