| Autor: |
Hugo G.J. Damstra, Josiah B. Passmore, Albert K. Serweta, Ioannis Koutlas, Mithila Burute, Frank J. Meye, Anna Akhmanova, Lukas C. Kapitein |
| Rok vydání: |
2022 |
| Popis: |
Expansion microscopy (ExM) is a powerful technique to overcome the diffraction limit of light microscopy by physically expanding biological specimen in three dimensions. Nonetheless, using ExM for quantitative or diagnostic applications requires robust quality control methods to precisely determine expansion factors and to map deformations due to anisotropic expansion. Here we present GelMap, a flexible workflow to introduce a fluorescent grid into pre-expanded hydrogels that scales with expansion and reports deformations. We demonstrate that GelMap can be used to precisely determine the local expansion factor and to correct for deformations without the use of cellular reference structures or pre-expansion ground truth images. Moreover, we show that GelMap aids sample navigation for correlative uses of expansion microscopy. Finally, we show that GelMap is compatible with expansion of tissue and can be readily implemented as a quality control step into existing ExM workflows. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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