Step-by-step Control by Time-of-flight Secondary Ion Mass Spectrometry of a Peptide Synthesis Carried Out on Polymer Beads
| Autor: | Jean Martinez, J.-L. Aubagnac, Christine Enjalbal, Robert Combarieu, C. Drouot, Pierre Fulcrand, Y. de Puydt |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
chemistry.chemical_classification
Chromatography Collision-induced dissociation Protein mass spectrometry Organic Chemistry Selected reaction monitoring Peptide Combinatorial chemistry Analytical Chemistry Secondary ion mass spectrometry Time of flight chemistry.chemical_compound chemistry Fragmentation (mass spectrometry) Peptide synthesis Spectroscopy |
| Zdroj: | Rapid Communications in Mass Spectrometry. 10:1509-1511 |
| ISSN: | 1097-0231 0951-4198 |
| Popis: | The use of time-of-flight secondary ion mass spectrometry (Tof-SIMS) for the step-by-step control of a peptide synthesis on a solid support is illustrated. The positive secondary ions, issuing from energetic fragmentation, are characteristic of the terminal groups of the step under investigation. The negative secondary ions result from the cleavage of the benzyl ester link between the polymer support and the first amino acid, and provide direct identification of the growing sequence. Tof-SIMS offers this unique analytical possibility without any preliminary separation of the peptide from its support. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text