Assignment of the gene encoding the catalytic subunit Cβ of CAMP-dependent protein kinase to the p36 band on chromosome

Autor:	Vidar Hansson, Mårten Sandberg, Richard Gagné, Karl-Heinz Grzeschik, Tore Jahnsen, Dominique Bérubé, Jacques Simard
Rok vydání:	1992
Předmět:	genomic DNA cDNA library Complementary DNA Protein subunit Genetics Chromosome Locus (genetics) Biology Protein kinase A Molecular biology Genetics (clinical) Southern blot
Zdroj:	Human Genetics. 88:653-657
ISSN:	1432-1203 0340-6717
Popis:	A cDNA for the human catalytic subunit (Cβ) of cAMP-dependent protein kinase (PKA) has been cloned from a testis cDNA library. In the present study, we have determined the chromosomal localization of this gene using a cDNA for Cβ as a probe. Southern blot analysis of genomic DNA from human/mouse cell hybrids revealed that the presence or absence of a 20-kbXbaI fragment, which hybridized with the Cβ probe, was concordant with the presence of human chromosome 1.In situ hybridization to metaphase chromosome confirmed the somatic cell hybrid data and regionally mapped the Cβ gene of PKA to the p36 band on chromosome 1.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4989cae15dfc8381bb4cf041dd24a8f2 https://doi.org/10.1007/bf02265292 Zobrazit plný text záznamu Full text from SpringerLink