The dependence of proteins’ distribution within polyelectrolyte microcapsules on pH of the medium

Autor:	L. I. Shabarchina, N. V. Apolonnik, D. A. Moshkov, I. M. Santalova, L. I. Kazakova, A. V. Dubrovskyi
Rok vydání:	2012
Předmět:	chemistry.chemical_classification biology Organic Chemistry Shell (structure) Analytical chemistry Capsule Peptide Biochemistry Polyelectrolyte law.invention Ferritin Isoelectric point chemistry Confocal microscopy law biology.protein Bovine serum albumin
Zdroj:	Russian Journal of Bioorganic Chemistry. 38:51-55
ISSN:	1608-330X 1068-1620
DOI:	10.1134/s1068162012010128
Popis:	The distribution of bovine serum albumin and ferritin within polyelectrolyte microcapsules was studied by transmission electron and confocal microscopy at the pH range 2–5. It was estimated that the protein’s distribution depends on the isoelectric point (pI) and first polyelectrolyte used for the preparation of the capsule shell. The peptide is placed in the bulk of capsule if the pH values of the medium are close to the isoelectric point of the protein and polycation was used as a first polyelectrolyte layer. If the first polyelectrolyte was polyanion, the protein is located near the internal surface of the shell. The protein is situated near the internal surface of the shell for both polyelectrolytes when pH is equal to pI.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4971536eb305e4bc2611057c6bd5638f https://doi.org/10.1134/s1068162012010128 Zobrazit plný text záznamu Full text from SpringerLink