Microbiological parameters of aggregates in typical chernozems of long-term field experiments
| Autor: | O. V. Kutovaya, V. I. Lazarev, A. K. Tkhakakhova, V. A. Kholodov, A. D. Zhelezova, N. V. Yaroslavtseva, B. M. Kogut, S. A. Garbuz |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
geography
geography.geographical_feature_category Soil test biology Steppe Microorganism Soil Science Soil science 04 agricultural and veterinary sciences 010501 environmental sciences biology.organism_classification 01 natural sciences Agronomy Microbial population biology 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Pcr analysis Chernozem 0105 earth and related environmental sciences Earth-Surface Processes Bacterial dna Archaea |
| Zdroj: | Eurasian Soil Science. 50:701-707 |
| ISSN: | 1556-195X 1064-2293 |
| Popis: | The changes in microbiological parameters of aggregates (1–2 mm) in typical chernozems under different land uses as dependent on the intensity and character of anthropogenic loads were studied with the help of the real-time polymerase chain reaction (PCR). The samples from the following long-term field experiments were examined: permanent black fallow, continuous cultivation of potato, 17-year-old unmanaged fallow after permanent black fallow, and annually mown reserved steppe. The soil samples were treated in two ways. In the first case, the samples were air-dried, sieved through the screens to separate aggregate fraction of 1–2 mm, and microbiological parameters were determined in this fraction. In the second case, the samples were frozen immediately after the sampling, and the aggregates of 1–2 mm were manually separated from the samples before the PCR analysis. It was shown that air-dry aggregates of chernozems could be used for the quantitative analysis of DNA of microbial community in comparative studies. According to the quantitative estimate of the content of DNA fragments from different phylogenetic groups, the bacterial community was most sensitive to the type of the soil use, and its restoration after the removal of extreme anthropogenic loads proceeded faster than that of other microorganisms. The content of archaeal DNA in the chernozem under the 17-year-old unmanaged fallow did not differ significantly from its content in the annually plowed chernozems. The changes in the content of micromycetal DNA related to anthropogenic load decrease were intermediate between changes in the contents of archaeal and bacterial DNA. |
| Databáze: | OpenAIRE |
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