| Popis: |
Out of 2942 blood serum samples from small ruminants of 10 flocks with a natural course of brucellosis caused by B. melitensis, 322 samples reacted with both antigens in the RID, of which 90 samples only with the O-PS M antigen (from B. melitensis), only with O-PS A-antigen (from B. abortus) reactive was not revealed. In healthy sheep immunized against brucellosis with the vaccine from strain 19 according to different schemes, only the O-PS M antigen was not found to react. Reaction with O-PS A- and M-antigens was observed in animals that were immunized twice subcutaneously at a dose of 40 billion mc. - after 2 months. after revaccination (60%), as well as in those reimmunized conjunctivally at a dose of 4 billion mc. according to the background of primary immunization subcutaneously at a dose of 40 billion mc. (10%) In animals immunized once or twice conjunctivally, reacting in RID with both antigens was not detected. Out of 2432 blood serum samples of small ruminants, 10 flocks with a brucellosis problem immunized against brucellosis with a vaccine from B.abortus strain 19 according to different schemes, 151 samples (6.2%) reacted positively with both O-PS antigens in RID with both O-PS antigens, of which only 86 samples (56.9%) reacted with O-PS M-antigen. The prevalence of indications of RID with O-PS M-antigen over RID with O-PS A-antigen (O-PS antigen made from Brucellae abortus) in small ruminants in one or another flock is characteristic of infection caused by brucellae melitensis at least in the absence, at least in the presence of the fact of immunization with a vaccine from the B. abortus 19 strain. RID with O-PS M-antigen is an objective indicator of epizootic danger and is able to differentiate brucellosis (B. melitensis) in small ruminants from vaccination-induced reactions (B. abortus 19). |
