Assessment of Different Regeneration and Washing Solutions in an Affinity Chromatography Procedure to Purify Plantibodies

Autor:	Sigifredo Padilla, Tatiana González, Déborah Geada, Mayra Wood, Tatiana Alvarez, Yenisley Medina, Rodolfo Valdés, Williams Ferro, Andrés Tamayo
Rok vydání:	2012
Předmět:	chemistry.chemical_compound Chromatography Affinity chromatography Chemistry Matrix stability Organic Chemistry Clinical Biochemistry Plantibody Citric acid Biochemistry Phosphoric acid Analytical Chemistry
Zdroj:	Chromatographia. 75:403-409
ISSN:	1612-1112 0009-5893
DOI:	10.1007/s10337-011-2168-2
Popis:	Purification efficiency is a great challenge in plantibody production. Several regeneration + washing solutions of ProSep®-vA Ultra matrix used in plantibody HB-01 purification were assessed in this study to increase matrix stability without affecting purity and to reduce costs. In conclusion, PHB-01 purity was always higher than 90%. Citric acid (100 mM); pH 3.0/0.1% Triton X-100 + 150 mM PBS; pH 8.0 and 250 mM phosphoric acid; pH 1.5 + 150 mM PBS; pH 8.0/0.1% Triton X-100 allowed increasing purification cycles up to 60 and antibody yield 1.4 and 1.3 fold, respectively, together with a significant reduction in plantibody purification cost.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::495174e51295626e2e181923f68077ba https://doi.org/10.1007/s10337-011-2168-2 Zobrazit plný text záznamu Full text from SpringerLink