High performance liquid chromatography to determine animal drug clenbuterol in pork, beef and hog liver
| Autor: | Shin-Shou Chou, Lin-Ying Chang, Deng-Fwu Hwang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pharmacology
Detection limit 0303 health sciences Chromatography 030309 nutrition & dietetics Chemistry 010401 analytical chemistry Extraction (chemistry) 01 natural sciences High-performance liquid chromatography 0104 chemical sciences Absorbance 03 medical and health sciences chemistry.chemical_compound Clenbuterol medicine Perchloric acid Diethyl ether Food Science medicine.drug |
| Zdroj: | Journal of Food and Drug Analysis. 13 |
| ISSN: | 2224-6614 |
| DOI: | 10.38212/2224-6614.2542 |
| Popis: | A semi-micro high performance liquid chromatography (HPLC) was developed to determine clenbuterol in pork, beef and hog liver. The procedure included extraction with 0.4 N perchloric acid, partitionally separation with diethyl ether, and then back-extraction with 0.2 M sulphuric acid. Analytical procedure was performed by HPLC with a Waters Cosmosil column 5C18-MS (2.0×150 mm), a mobile phase of 0.05 M NaH2PO4 (pH3.0)/acetonitrile solution (80/20, v/v) and absorbance at 212 nm. The recoveries of clenbuterol in pork, beef and hog liver spiked with standards of 0.005~0.01 ppm were 80.9~90.6%. The detection limit of clenbuterol in tested samples was 0.0001 ppm, which is lower than the officially allowed level. The equation was linear for detecting 0.0002~0.001 ppm clenbuterol. The method was validated to be usable for market samples. Three out of 150 samples were detected to contain clenbuterol (0.000l~0.00015 ppm), which were less than both JECFA and Department of Health regulation levels (hog liver 0.0006 ppm and beef 0.0002 ppm). |
| Databáze: | OpenAIRE |
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