Ratiometric sensing of metabolites using dual-emitting ZnS:Mn 2+ quantum dots as sole luminophore via surface chemistry design
| Autor: | Yunsheng Xia, Gong Suqin, Wenliang Gu, Yunlong Zhou |
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| Rok vydání: | 2017 |
| Předmět: |
Detection limit
Ammonium bromide Chemical substance Biomedical Engineering Biophysics Analytical chemistry 02 engineering and technology General Medicine 010402 general chemistry 021001 nanoscience & nanotechnology Photochemistry 01 natural sciences 0104 chemical sciences chemistry.chemical_compound chemistry Linear range Quantum dot Electrochemistry Luminophore Molecule 0210 nano-technology Selectivity Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 90:487-493 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2016.10.074 |
| Popis: | We herein present an effective and versatile platform for ratiometric sensing of metabolites using intrinsically dual-emitting ZnS:Mn2+ quantum dots (QDs) as sole reporter. To avoid notoriously non-specific interactions, a special triple-layer "filter screen" around the inorganic QD core is rationally constructed, which is made of oleic acid, cetyltrimethyl ammonium bromide and bio-enzymes. In the presence of the analytes, the in-situ enzymatic H2O2 molecules diffuse and pass through the "filter screen" along the molecule interspace, which then reacts with the inorganic core and leads to more dramatically quenching of the Mn2+ emission. The ratiometric signal readout is so distinct that can be observed by naked eyes (from orange to violet). In contrast, various coexisting bio-molecules, due to larger size, are well prevented from penetrating the filter screen by steric hindrance effect. So, various potential interfering substances do not disturb the assay. Under optimal conditions, five kinds of the corresponding substrates, namely glucose, cholesterol, lactate, xanthine and uric acid are well quantified by the emission intensity ratio of I470/I615, and the linear ranges are 0.1-200µM, 0.1-200µM, 1-200µM, 1-200µM and 1-200µM, respectively. The detection limits can even reach quasi-picomole levels. Because of favorable analytical performances (excellent selectivity, appropriate sensitivity and broad linear range), the proposed system can direct assay the analytes in blood without any sample pre-treatment. |
| Databáze: | OpenAIRE |
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