Morphine-induced Cognitive Dysfunction Model in Zebrafish
| Autor: | Ishwar S. Parhar, Pek Yee Tang, Wen-Yang Png, Siew-Ying Mok, Satoshi Ogawa |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Startle response animal structures medicine.diagnostic_test biology business.industry fungi Cognition medicine.disease biology.organism_classification 03 medical and health sciences Neural Pathway 030104 developmental biology 0302 clinical medicine Schizophrenia Morphine medicine Biological neural network Habituation business Neuroscience Zebrafish 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | 2020 IEEE-EMBS Conference on Biomedical Engineering and Sciences (IECBES). |
| Popis: | The neural pathway of opioid use plays a crucial role in drug abuse, cognitive alteration, and abnormal change in neurobehavior. This study aims to investigate the effect of morphine on changes in cognitive aspect through assessment of the startle habituation response in the zebrafish model. Zebrafish embryos were exposed to 0.8 µM morphine for 5 days, starting from 5-hour post-fertilisation (hpf). On 6-day post-fertilisation (dpf), the larvae were transferred to the custom startle apparatus for assessment. The recorded videos were analysed using Toxtrac software. From the analysis, morphine-treated larvae showed impairment in the habituation response, with excessive startle response and non-consistent decrement in habituation. Besides, chaotic movements and preference towards peripheral zone in the larvae treated with morphine also signifying anxiety. We postulate that the morphine regulated pathway are involved in the cognitive alteration and abnormal behaviour of morphine-treated zebrafish. Future studies are required to associate the morphine-induced behaviour to the neural circuitry and firing activities. |
| Databáze: | OpenAIRE |
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