Tangential Flow Filtration with or Without Subsequent Bind-Elute Size Exclusion Chromatography for Purification of Extracellular Vesicles
| Autor: | Joel Z. Nordin, R. Beklem Bostancioglu, Giulia Corso, Samir El Andaloussi |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Chromatography Chemistry Elution Vesicle Size-exclusion chromatography 02 engineering and technology 021001 nanoscience & nanotechnology Extracellular vesicles Cross-flow filtration 03 medical and health sciences 030104 developmental biology Ultracentrifuge Purification methods 0210 nano-technology |
| Zdroj: | Target Identification and Validation in Drug Discovery ISBN: 9781493991440 |
| Popis: | Extracellular vesicles (EVs) have gained increased attention over the last decade due to their potential as biomarkers and therapeutic entities. However, the characterization and development of EV research has been hampered by the lack of sufficiently effective purification methods. Several concerns have been raised toward the gold standard purification method ultracentrifugation, such as operator-dependent yields, crushing and aggregation of vesicles, poor scalability, and relative lack of purity. Here, we describe, in details, the use of an alternative purification technique: tangential flow filtration with or without subsequent bind-elute size exclusion chromatography that we have previously shown to be reproducible and scalable for purification of EVs. |
| Databáze: | OpenAIRE |
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