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OBJECTIVE Cervical cancer(CCA) is the second commonest malignancy among female all over the world, and present clinical treatments cannot solve the problems of high metastasis and chemotherapy-resistant in CCA. This study starts with RNA-seq analysis and aims to investigate the possibility of secretory protein MMP3 as a new diagnosis and therapeutic target in CCA. METHODS Through conjoint analysis of gene expression data as well as survival rate data, we explored the potential secretary proteins associated with CCA carcinogenesis and advance and verify the expression changes in serum of clinical patients. We knockdown or overexpress the secretory proteins then explore its influence on biological function of CCA cells. Cell viabilities and apoptosis levels are detected using CCK-8 kit and TUNEL staining assay respectively, the expression of apoptosis related proteins was verified by western blot. RESULTS By cross-analysis of The Cancer Genome Atlas (TCGA) database and MetazSecKB database, MMP3 gene was most significantly upregulated in CCA patients. Also, MMP3 protein was remarkably increased in the serum of clinical CCA patients and decreased after receiving treatment. Overexpression of MMP3 in HT-3 cells or culturing new cells using the supernatant of the medium after MMP3 overexpression could increase cell viability (p p |
