Expression of the histamine receptor in Xenopus oocyte and its application to the histamine sensor
| Autor: | Jun Otomo, Shinichi Fukuzono, Nukada Toshihide, Tomoko Takeshita, Takeshi Sakamoto |
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| Rok vydání: | 2000 |
| Předmět: |
Messenger RNA
biology Chemistry Metals and Alloys Xenopus Condensed Matter Physics Oocyte biology.organism_classification Mast cell Surfaces Coatings and Films Electronic Optical and Magnetic Materials Cell biology Cell membrane Histamine receptor chemistry.chemical_compound medicine.anatomical_structure Materials Chemistry Chloride channel medicine Electrical and Electronic Engineering Instrumentation Histamine |
| Zdroj: | Sensors and Actuators B: Chemical. 66:19-21 |
| ISSN: | 0925-4005 |
| Popis: | We developed a receptor-mediated biological sensor for real time detection of histamine derived from granules of mast cell and basophils. A human histamine receptor gene was cloned by polymerase chain reaction and its mRNA was injected into Xenopus oocyte. After incubation for 2–3 days, the histamine receptor was expressed in the cell membrane of the oocyte. When the oocyte was activated by histamine, a calcium-activated chloride channel was opened, and the chloride ions in the oocyte flowed to the outside. The chloride ion current intensity was correlated with the histamine concentration. The histamine sensitivity of the oocyte was sufficient to allow comparison with other methods histamine analyses, such as enzyme immunoassay or HPLC analysis. The oocyte sensor system described here is a new chemical sensor system using receptor-mediated biological reactions. |
| Databáze: | OpenAIRE |
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