| Popis: |
In this study, the effects of adding hydrogen peroxide (H2O2), nicotinamide adenine dinucleotide phosphate (NADPH) and phenylalanine (substrates of specific oxidases) on ram sperm motility were investigated. Fresh Awassi ram sperm were incubated at 37 oC for 30, 60, 120 and 180 minutes with (100 μM and 1 mM) or without H2O2, NADPH and phenylalanine in egg yolk Tris (EYT) and Tyrode albumen lactate (TAL) media. Sperm motility was assessed using computer aided sperm analysis (CASA). Following sperm incubation with 100 μM of H2O2 at different time intervals, no significant changes (P > 0.05) were observed compared to control in percent of motility (MOT %), progressive motility (PMOT %) and average path velocity (VAP). Incubation with 1 mM H2O2 led to a significant (P < 0.05) decrease in all motility parameters. Higher values (P < 0.05) of CASA parameters (especially PMOT % and VAP) were achieved when 1 mM of NADPH and phenylalanine were added in the two media. We concluded that fresh Awassi ram sperm appears to support certain levels of hydrogen peroxide in both TAL and EYT media and this may reflect an antioxidant capacity. Moreover, the addition of oxidase substrates in 1 mM concentration to ram semen media could be useful as stimulating additives for ram sperm motility. |
