Biosynthesis of Gold and Silver Nanoparticles Using Extracts of Callus Cultures of Pumpkin (Cucurbita maxima)
| Autor: | Tapobrata Panda, R. Indira Iyer |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Materials science Biomedical Engineering Nanoparticle Bioengineering 02 engineering and technology 01 natural sciences Silver nanoparticle chemistry.chemical_compound Zeta potential General Materials Science Fourier transform infrared spectroscopy biology fungi food and beverages General Chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics biology.organism_classification chemistry Colloidal gold Callus 0210 nano-technology Methylene blue Cucurbita maxima 010606 plant biology & botany Nuclear chemistry |
| Zdroj: | Journal of Nanoscience and Nanotechnology. 18:5341-5353 |
| ISSN: | 1533-4880 |
| DOI: | 10.1166/jnn.2018.15378 |
| Popis: | The potential of callus cultures and field-grown organs of pumpkin (Cucurbita maxima) for the biosynthesis of nanoparticles of the noble metals gold and silver has been investigated. Biosynthesis of AuNPs (gold nanoparticles) and AgNPs (silver nanoparticles) was obtained with flowers of C. maxima but not with pulp and seeds. With callus cultures established in MS-based medium the biogenesis of both AuNPs and AgNPs could be obtained. At 65 °C the biogenesis of AuNPs and AgNPs by callus extracts was enhanced. The AuNPs and AgNPs have been characterized by UV-visible spectroscopy, TEM, DLS and XRD. Well-dispersed nanoparticles, which exhibited a remarkable diversity in size and shape, could be visualized by TEM. Gold nanoparticles were found to be of various shapes, viz., rods, triangles, star-shaped particles, spheres, hexagons, bipyramids, discoid particles, nanotrapezoids, prisms, cuboids. Silver nanoparticles were also of diverse shapes, viz., discoid, spherical, elliptical, triangle-like, belt-like, rod-shaped forms and cuboids. EDX analysis indicated that the AuNPs and AgNPs had a high degree of purity. The surface charges of the generated AuNPs and AgNPs were highly negative as indicated by zeta potential measurements. The AuNPs and AgNPs exhibited remarkable stability in solution for more than four months. FTIR studies indicated that biomolecules in the callus extracts were associated with the biosynthesis and stabilisation of the nanoparticles. The synthesized AgNPs could catalyse degradation of methylene blue and exhibited anti-bacterial activity against E. coli DH5α. There is no earlier report of the biosynthesis of nanoparticles by this plant species. Callus cultures of Cucurbita maxima are effective alternative resources of biomass for synthesis of nanoparticles. |
| Databáze: | OpenAIRE |
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