Heterogeneity and composition of .KAPPA.-casein from bovine colostrum

Autor: Bong Sun Park, Fumio Ibuki, Hiroshi Doi, Masao Kanamori
Rok vydání: 1980
Předmět:
Zdroj: Agricultural and Biological Chemistry. 44:813-820
ISSN: 1881-1280
0002-1369
Popis: The heterogeneity and chemical composition were investigated in κ-casein from colostrum. The acid casein was obtained from four different Holstein cow colostra. The yield of acid casein from colostrum was higher than that from normal milk. κ-Casein from colostrum was prepared by the gel filtration method of Yaguchi et al. The gel filtration profiles differed among the four colostrum acid caseins.Colostrum κ-casein was fractionated on a DEAE-cellulose column into one nonadsorbed and six adsorbed fractions with increasing salt concentration. Six adsorbed fractions had the same molecular weight and stabilizing ability for αs1-casein in the presence of calcium ion. The amino acid composition and the phosphorus content of the adsorbed fractions were identical, but fractions eluted with high salt concentrations had more carbohydrates (galactose, sialic acid, glucosamine, galactosamine). Colostrum κ-casein was characterized by a higher content of carbohydrate moiety in comparison with normal κ-casein. Also gluco...
Databáze: OpenAIRE