| Autor: |
Melanie Bahlo, Moses Laman, Alyssa E. Barry, Abebe A. Fola, Ivo Mueller, Zahra Razook, Somya Mehra, Didier Menard, Digjaya Utama, Leanne J. Robinson, Dulcie Lautu-Gumal, James W. Kazura, Brittany Gilchrist |
| Rok vydání: |
2020 |
| Předmět: |
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| Popis: |
Malaria parasite genomes have been generated predominantly using short read sequencing technology which can be slow, requires advanced laboratory training and does not adequately interrogate complex genomic regions that harbour important malaria virulence determinants. The portable Oxford Nanopore Technologies MinION platform generates long reads in real time and may overcome these limitations. We present compelling evidence that Nanopore sequencing delivers valuable additional information for malaria parasites with comparable data fidelity for single nucleotide variant (SNV) calls, compared to standard Illumina whole genome sequencing. We demonstrate this through sequencing of purePlasmodium falciparumDNA, mock infections and natural isolates. Nanopore has low error rates for haploid SNV genotyping and identifies structural variants (SVs) not detected with short reads. Nanopore genomes are directly comparable to publically available genomes and produce high quality end to end chromosome assemblies. Nanopore sequencing will expedite genomic surveillance of malaria and provide new insights into parasite genome biology. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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