EST–SSR markers for asparagus genetic diversity evaluation and cultivar identification

Autor: Marco Caruso, Claire T. Federici, Mikeal L. Roose
Rok vydání: 2007
Předmět:
Zdroj: Molecular Breeding. 21:195-204
ISSN: 1572-9788
1380-3743
DOI: 10.1007/s11032-007-9120-z
Popis: Simple sequence repeat (SSR) markers generated from expressed sequence tag (EST) sequences represent useful tools for genotyping and their development is relatively easy because of the public availability of EST databases. We report design and application of EST–SSRs to assess the level of genetic diversity among thirty-five asparagus cultivars and to fingerprint DePaoli, a new variety released by University of California, Riverside. DNA was isolated from bulks of pooled cladophylls coming from five plants of each variety to reduce the number of DNA extractions and PCR reactions. Allele frequencies were estimated from the intensity of the bands in two bulks and two individual plant samples for each variety. Although asparagus varieties derive from a limited germplasm pool, eight EST–SSR loci differentiated all of the analyzed cultivars. Moreover, UPGMA (unweighted pair group method with arithmetic mean) and neighbor-joining trees, as well as principal components analysis separated the cultivars into clusters corresponding to the geographical areas where they originated.
Databáze: OpenAIRE
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