Blood serum and follicular fluid relaxin: a pilot study of the hormone effects on ovarian function and fertilization efficiency
| Autor: | Natalia N. Tkachenko, Olga V. Kosyakova, Olesya N. Bespalova, Igor Yu. Kogan, Valentina L. Borodina, Elena A. Lesik, Irina D. Mekina, Alexander M. Gzgzyan, Evgenia M. Komarova, Yulia P. Milyutina, Valeriya A. Zagaynova |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Relaxin Infertility endocrine system 030219 obstetrics & reproductive medicine In vitro fertilisation business.industry medicine.drug_class medicine.medical_treatment media_common.quotation_subject Obstetrics and Gynecology medicine.disease Follicular fluid Andrology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Human fertilization Blood serum medicine Gonadotropin business Ovulation hormones hormone substitutes and hormone antagonists media_common |
| Zdroj: | Journal of obstetrics and women's diseases. 69:59-68 |
| ISSN: | 1683-9366 1684-0461 |
| DOI: | 10.17816/jowd69559-68 |
| Popis: | Hypothesis/aims of study. To date, one of the most important avenues of research in the field of reproductive medicine is the searching for new biochemical markers of oocyte quality and the prediction of the effectiveness of in vitro fertilization (IVF) protocols. The aim of this study was to assess the effect of relaxin levels in blood serum and follicular fluid on the efficiency of ovulation stimulation, fertilization, and characteristics of the embryos. Study design, materials and methods. This prospective randomized cohort study included 11 patients undergoing infertility treatment in a superovulation stimulation protocol using gonadotropin-releasing hormone antagonists. Age, body mass index, hormonal status, ovarian response, endometrial thickness and structure, the number and quality of oocytes and embryos, as well as fertilization efficiency were assessed. The level of relaxin in blood serum and follicular fluid samples was determined on the day of transvaginal follicle puncture using enzyme immunoassay. Results. A correlation between follicular fluid relaxin levels and body mass index, age, the number of oocytes, and their fertilization efficiency (p 0.05) was established. Changes in follicular fluid relaxin level were revealed depending on the gonadotropin preparations (p 0.05) and triggers of final maturation of oocytes (p 0.05). The tendency of the effect of gonadotropin doses on circulating relaxin levels, and of the hormone itself on endometrial thickness and the quality of oocytes was determined. Conclusion. Determination of the relaxin concentration can be considered as a promising method for predicting the result of ovarian stimulation and the efficiency of fertilization in IVF protocols. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|