| Autor: |
M. V. Pivkin, N V Zhuravleva, P A Luk'ianov |
| Rok vydání: |
2004 |
| Předmět: |
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| Zdroj: |
Applied Biochemistry and Microbiology. 40:448-453 |
| ISSN: |
0003-6838 |
| DOI: |
10.1023/b:abim.0000040666.13870.52 |
| Popis: |
Among the ten strains of marine fungi studied, the mycelial fungus Phoma glomerata showed maximum potency in producing N-acetyl-beta-D-glucosaminidase. The conditions for fungal growth and enzyme biosynthesis were evaluated. N-acetyl-beta-D-hexosaminidase was isolated from the culture liquid of Phoma glomerata by ion-exchange chromatography (on DEAE-cellulose and DEAE-Sephacell) and gel filtration (on Toyopearl HW-55) with a yield of 35%; the enzyme, purified 36.4-fold, had a molecular weight of 20 kDa. The homogeneity of the enzyme was confirmed by gel filtration and SDS-PAGE. Transglycosylation reactions catalyzed by the enzyme produced N-acetyl-D-glucosamine and N-acetyl-D-galactosamine with respective yields of 38 and 46%. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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