| Autor: |
Ruth Knox, Emily Kempa, Reynard Spiess, Christian Schnepel, Sabine Flitsch, Perdita Barran, Nicholas Turner |
| Rok vydání: |
2022 |
| Popis: |
Ambient ionization coupled to mass spectrometry has the advantages of minimal requirements for sample preparation prior to analysis which renders it suitable for high throughput screening. We present a protocol that permits the application of this method in routine biotechnology and chemical biology laboratories which are using engineered enzymes to produce target compounds from substrates. We show how DESI-MS can be used to directly analyse the activity of biotransformations from crude cell lysate which we term DiBT-MS, this method is 10-1000 times faster than LC-MS and uses far less solvent. This protocol demonstrates the impact of solvent spray composition on ionization efficiency of the target analyte, the benefits of a nylon membrane slide and the reusability of sample slides in multiple experiments. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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