| Popis: |
This paper presents the design and performance of a multiplexed microfluidic system capable of performing six immunoassays simultaneously, based upon on-chip mixing, reaction and affinity capillary electrophoresis. Utilizing a single point, galvanic-scanner based detection system with detection limits of 30 pM for fluorescein (FL), both qualitative and quantitative immunoassays have been performed. Factors governing good quality, quantitative measurements are discussed. The results show a complete estradiol (E2) immunoassay, including calibration, can be performed on chip in about 1 minute. |
