A Seasonal and Age-Related Study of Interstitial Cells in the Pineal Gland of Male Viscacha(Lagostomus maximus maximus)
| Autor: | Fabian Mohamed, Fabricio Ivan Busolini, Gabriela Judith Rosales, Veronica Palmira Filippa |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Histology food.ingredient Vimentin 03 medical and health sciences Pineal gland 0302 clinical medicine food Internal medicine medicine Perivascular space Ecology Evolution Behavior and Systematics Lagostomus biology Colocalization Viscacha biology.organism_classification 030104 developmental biology medicine.anatomical_structure Endocrinology Cytoplasm biology.protein Immunohistochemistry Anatomy 030217 neurology & neurosurgery Biotechnology |
| Zdroj: | The Anatomical Record. 300:1847-1857 |
| ISSN: | 1932-8486 |
| Popis: | The pineal gland of viscacha exhibits histophysiological variations throughout the year, with periods of maximal activity in winter and minimal activity in summer. The aim of this study was to analyze the interstitial cells (IC) in the pineal gland of male viscachas in relation to season and age. The S-100 protein, glio-fibrillary acidic protein (GFAP), and vimentin were detected in adult and immature animals by immunohistochemistry (IHC). Double-IHC was also performed. The S-100 protein was localized within both, IC nucleus and cytoplasm. GFAP was present only in the cytoplasm. Vimentin was expressed in some IC, besides endothelial cells, and perivascular spaces. In the adult males, the morphometric parameters analyzed for the S-100 protein and GFAP exhibited seasonal variations with higher values of immunopositive area percentage in winter and lower values in summer, whereas the immature ones showed the lowest values for all the adult animals studied. Colocalization of S-100 protein and GFAP was observed. The IC exhibited differential expression for the proteins studied, supporting the hypothesis of the neuroectodermal origin. The IC generate an intraglandular communication network, suggesting its participation in the glandular activity regulation processes. The results of double-IHC might indicate the presence of IC in different functional stages, probably related to the needs of the cellular microenvironment. The morphometric variations in the proteins analyzed between immature and adult viscachas probed to be more salient in the latter, suggesting a direct relationship between the expression of the S-100 protein and GFAP, and animal age. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text