| Autor: |
Maria Antonietta Madeddu, Angela Ingianni, Sandro Dessì, Raffaello Pompei, Marta Quartuccio, A Sanna |
| Rok vydání: |
2007 |
| Předmět: |
|
| Zdroj: |
American Journal of Infectious Diseases. 3:159-164 |
| ISSN: |
1553-6203 |
| Popis: |
The isolation and identification of Listeria monocytogenes in processed meat samples by a combined cultural-molecular method is described. It allows the identification of Listeria strains by means of a hybridization technique with a specific DNA probe directed to the listerial internalin gene. The specificity of this method was found to be 100% and sensitivity was as low as 1 CFU/2.5 g of food sample. A total of 278 meat samples were tested in comparison with PCR and conventional cultural assays. A total of 42 (15.4%) L. monocytogenes were detected. PCR analysis gave 3 false negative results and culture failed to detect the Listeria in 5 cases. With this cultural-molecular method the identification and quantitative detection of L. monocytogenes were achieved within 36 hours and no false positive or negative tests were obtained, thus fitting most food industry requirements. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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