Application of microbes and microbial esterases to the preparation of optically active N-acetylindoline-2-carboxylic acid

Autor:	Oreste Ghisalba, Gerardo M. Ramos Tombo, Hans-Peter Schär
Rok vydání:	1987
Předmět:	chemistry.chemical_classification biology Stereochemistry Carboxylic acid Pseudomonas biology.organism_classification Esterase General Biochemistry Genetics and Molecular Biology Amino acid Enzyme catalysis Hydrolysis chemistry General Agricultural and Biological Sciences Sludge Bacteria
Zdroj:	Agricultural and Biological Chemistry. 51:1833-1838
ISSN:	1881-1280 0002-1369
DOI:	10.1271/bbb1961.51.1833
Popis:	Methylotrophic bacteria, isolated from soil samples or from sewage sludge, proved to be useful sources of esterases for catalyzing the enantioselective hydrolysis of racemic N-acetyl-indoline-2-carboxylic acid methyl ester (7) to the corresponding (25) or (2R)-N- acetyl amino acid (6) with high optical yields. From the DMF-utilizer Pseudomonas DMF 5/8 and the methanol-utilizer Isolate EE 210, the corresponding esterases were isolated. Reactions with whole cells as well as with the purified enzymes are described.
Databáze:	OpenAIRE
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