Microsensors in Icy Environments to Detect Microbial Activities
| Autor: | Markus Dieser, Christine M. Foreman, Matthew McGlennen, Matthew Driesler, Michael Neubauer, Stephan Warnat |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Flavobacterium sp Materials science 010504 meteorology & atmospheric sciences 030306 microbiology Focused Impedance Measurement Mechanical Engineering Impedance spectrum 01 natural sciences Temperature measurement Dielectric spectroscopy 03 medical and health sciences Bacterial isolate Electrical and Electronic Engineering Biological system Spectroscopy Electrical impedance 0105 earth and related environmental sciences |
| Zdroj: | Journal of Microelectromechanical Systems. 29:853-859 |
| ISSN: | 1941-0158 1057-7157 |
| DOI: | 10.1109/jmems.2020.3012420 |
| Popis: | Electrochemical techniques such as impedance spectroscopy offer a non-invasive approach to monitor microorganisms in natural and engineered environments. Here, we present data on the use of microfabricated impedance spectroscopy sensors for the detection of microbes in icy environments. Under controlled laboratory settings, the effects of different cell concentrations of the Antarctic isolate Flavobacterium sp. ANT 11 on impedance spectroscopy was investigated at ambient temperature (22°C) and -10°C. Results show that varying cell concentrations of the bacterial isolate generate unique spectral responses that vary with temperature. Subsequent tests on the performance of microfabricated impedance sensors on natural microbial communities in icy environments in the Beartooth Mountains, WY, USA, confirmed laboratory findings. Different environmental samples at varying microbial concentrations in liquid, semi-frozen (slushy), or frozen states generated discrete impedance spectra. While caution is advised to generalize these results due to the potential contribution of unknown environmental variables, our data provide fertile territory for research in cryo-microbiology, as information on the applicability of impedance spectroscopy under frozen conditions is limited. [2020-0150] |
| Databáze: | OpenAIRE |
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