Hormone injections enhance the tolerance of land-locked ayu spermatozoa to cryopreservation

Autor:	Hiromi Ohta, Ken-ichi Yokoi, Mitsugi Gouda, Kenji Takii, Tomonori Kuwada, Yoshinori Nishiguchi, Shinichi Yamamoto, Koji Yamane
Rok vydání:	2009
Předmět:	endocrine system medicine.medical_specialty Hormone injections medicine.medical_treatment Significant difference Motility Aquatic Science Biology Sperm Cryopreservation Steroid Endocrinology Internal medicine medicine Plecoglossus altivelis Hormone
Zdroj:	Aquaculture Research. 40:1893-1898
ISSN:	1365-2109 1355-557X
DOI:	10.1111/j.1365-2109.2009.02311.x
Popis:	We evaluated the effects of maturation-stimulating hormones on the post-thaw motility of land-locked form ayu (Plecoglossus altivelis) spermatozoa. Male ayu were administered three intraperitoneal injections of either salmon pituitary extract (SPE; 0.2 or 0.6 mg g−1 BW day−1) or of 17, 20β-dihydroxy-4-pregnen-3-one (DHP; 2 or 10 μg g−1 BW day−1), the maturation-inducing steroid (MIS) in ayu. Before cryopreservation, the motility of spermatozoa of the SPE- and DHP-treated groups was significantly higher than that of the control group. Similarly, the comparative post-thaw motility (presented as a percentage of the motility obtained before cryopreservation) was significantly higher in the SPE group than in the control; however, there was no significant difference between the DHP group and the control. The effect of SPE and DHP on pre- and post-cryopreservation motility was not dose dependent. Our results suggest that the hormone(s) present in salmon pituitary are effective in enhancing the tolerance of ayu sperm cells to cryopreservation and that the MIS (DHP) is not involved in this process.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4871d34a8ed2715e32869d275ffefe77 https://doi.org/10.1111/j.1365-2109.2009.02311.x Zobrazit plný text záznamu