Cloning and different expression of ATP synthase genes between propargite resistant and susceptible strains of Tetranychus cinnabarinus (Acarina: Tetranychidae)
| Autor: | Da Aisi, Chai Jianping, Kuanyu Zheng, Jiang Xiujun, Xie Daoyan, Yanjie Luo, Shao-xiang Li, Jing Ni, Yang Zhenguo |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cloning biology ATP synthase Acaricide ATPase biology.organism_classification 01 natural sciences Propargite Microbiology 010602 entomology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Spider mite Insect Science Gene expression biology.protein Gene |
| Zdroj: | Journal of Asia-Pacific Entomology. 21:402-407 |
| ISSN: | 1226-8615 |
| Popis: | The carmine spider mite, Tetranychus cinnabarinus (Boisduval), is a serious phytophagous mite damaging important crops and can rapidly develop resistance to acaricides. Mitochondrial ATP synthase (F1F0 ATP synthase) is an important target site of acaricides. The role of ATP synthase in acaricide resistance remains unclear at the molecular level. In this study, twelve full-length cDNAs of ATP synthase genes were cloned and characterized from T. cinnabarinus and their expression levels were determined for both progargite-resistant and susceptible strains. The effect of propargite exposure on gene expression was also evaluated. Analyses of gene expression revealed that TcATPsynU-2, TcATPsynF0-2 and TcATPsynF0-4 were significantly down-regulated in the progargite-resistant strain. TcATPsynF0-2 and TcATPsynF0-4 had a strong response to progargite exposure. The results suggest that lower levels of TcATPsynU-2, TcATPsynF0-2 and TcATPsynF0-4 expression might be related to propargite-resistance observed in the resistant T. cinnabarinus. This is the first attempt to identify specific ATPase genes involved in propargite resistance in T. cinnabarinus. |
| Databáze: | OpenAIRE |
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