Expression of a Functional FcεRI on Rat Eosinophils and Macrophages

Autor:	André Capron, Monique Capron, David Dombrowicz, Brigitte Quatannens, J P Papin
Rok vydání:	2000
Předmět:	Cell type biology medicine.diagnostic_test Immunology CD23 Immunoglobulin E biology.organism_classification Flow cytometry Cell biology Downregulation and upregulation Western blot biology.protein medicine Immunology and Allergy Schistosoma mansoni Receptor
Zdroj:	The Journal of Immunology. 165:1266-1271
ISSN:	1550-6606 0022-1767
DOI:	10.4049/jimmunol.165.3.1266
Popis:	Besides its crucial role in type I hypersensitivity reactions, IgE is involved in anti-parasite immunity. This role has been clearly demonstrated in both human and rat schistosomiasis, but remains controversial in the mouse. Since the cellular distribution of the high affinity IgE receptor, FcεRI, differs in humans and mice, it might explain the differences in effector function of IgE between the two species. In humans, eosinophils and macrophages induce IgE-dependent cytotoxicity toward Schistosoma mansoni larvae, which involves FcεRI in the case of eosinophils. In the present study, we have investigated the expression and function of FcεRI in rat eosinophils and macrophages. We demonstrate, by flow cytometry, fluorescence microscopy, and Western blot analysis, that in rats, as in humans, a functional αγ2 trimeric FcεRI is expressed on eosinophils and macrophages. We also show that these two cell types can induce IgE-mediated, FcεRI-dependent cellular cytotoxicity toward schistosomula. These results thus provide a molecular basis for the differences observed between rat and mouse regarding IgE-mediated anti-parasite immunity.
Databáze:	OpenAIRE
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