Acetylcholine induces intracellular Ca2+ oscillations and nitric oxide release in mouse brain endothelial cells

Autor:	Guerra, Germano, Zuccolo, Estella, Lim, Dmitry, Rosti, Vittorio, D’Angelo, Egidio, Lucariello, Angela, Perna, Angelica, Tafuri, Domenico, Moccia, Francesco
Jazyk:	angličtina
Rok vydání:	2017
Předmět:	Mouse brain microvascular endothelial cells bEND5 cells acetylcholine nitric oxide Ca2+ signaling intracellular Ca2+ oscillations inositol-1 5-trisphosphate receptors store-operated Ca2+ entry Orai2
DOI:	10.13128/ijae-21499
Popis:	Basal forebrain neurons increase cortical blood flow by releasing acetylcholine (Ach), which stimulates endothelial cells (ECs) to produce the vasodilating gasotransmitter, nitric oxide (NO). Surprisingly, the mechanism whereby Ach induces NO synthesis in brain microvascular ECs is unknown. An increase in intracellular Ca2 + concentration recruits a multitude of endothelial Ca2 +-dependent pathways, such as Ca2 +/calmodulin endothelial NO synthase (eNOS). The present investigation sought to investigate the role of intracellular Ca2 + signaling in Ach-induced NO production in bEnd5 cells, an established model of mouse brain microvascular ECs, by conventional imaging of cells loaded with the Ca2+-sensitive dye, Fura-2/AM, and the NO-sensitive fluorophore, DAF-DM diacetate. Overall, our data shed novel light on the molecular mechanisms whereby neuronally-released Ach controls neurovascular coupling in blood microvessels. Italian Journal of Anatomy and Embryology, Vol. 122, No. 1 (Supplement) 2017
Databáze:	OpenAIRE
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