On the non-enzymatic liberation of limonin and C17-epilimonin from limonin-17-β-d-glucopyranoside in orange juice

Autor: Anneke Glabasnia, Thomas Hofmann
Rok vydání: 2008
Předmět:
Zdroj: European Food Research and Technology. 228:55-63
ISSN: 1438-2385
1438-2377
Popis: Limonin is well-known to induce the so-called “delayed” bitterness which is developing during standing and/or processing of orange juice. The objective of this study was to investigate the hydrolytic liberation of limonin from its precursor limonin-17-β-d-glucopyranoside in aqueous model solutions and orange juice samples. Quantitative model studies on the degradation of limonin-17-β-d-glucopyranoside under acidic conditions as well as during storage of orange juice revealed the formation of limonin besides a second hydrolysis product of yet unknown structure. After isolation, the structure of that compound was unequivocally determined for the first time as the C17-epimer of limonin, named C17-epilimonin. In order to investigate the generation of these triterpenoid lactones during storage as well as heat-treatment of orange juice, freshly squeezed orange juice was stored for up to 4 weeks at 4 and 20 °C or was heated at 70 and 100 °C and, then, both limonoids were quantitatively determined by means of high performance liquid chromatography-mass spectrometry/mass spectrometry (multiple reaction monitoring). The data obtained showed first evidence for the use of the C17-epilimonin/limonin ratio as a suitable marker for the analytical determination of the thermal input applied during processing orange juice products.
Databáze: OpenAIRE
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