Model-based operational optimisation of a microbial bioprocess converting terephthalic acid to biomass
| Autor: | Kevin E. O’Connor, Niall Beagan, Ioscani Jimenez del Val |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Terephthalic acid
0303 health sciences Environmental Engineering biology Chemistry Pseudomonas Biomedical Engineering Biomass Substrate (chemistry) Bioengineering 010501 environmental sciences Bacterial growth biology.organism_classification Pulp and paper industry 01 natural sciences 03 medical and health sciences chemistry.chemical_compound 13. Climate action Carbon source Polyethylene terephthalate Bioprocess 030304 developmental biology 0105 earth and related environmental sciences Biotechnology |
| Zdroj: | Biochemical Engineering Journal. 158:107576 |
| ISSN: | 1369-703X |
| Popis: | Polyethylene terephthalate (PET) is a major contributor to plastic pollution due to its widespread presence in synthetic fibres. As an intermediate in the chemical recycling of PET, disodium terephthalate (Na2TA) can be harnessed as a cheap microbial substrate for the cultivation of Pseudomonas sp. GO16. Here, we present the model-based optimisation of a liquid feeding regime with the purpose of improving the automation and operational ease of Na2TA conversion into Pseudomonas sp. GO16 biomass. The model was parameterised and validated using data from dynamic liquid-phase and solid-phase feeding experiments. The validated model identified sodium ion accumulation as a key determinant of bioprocess performance and was used to design a liquid-phase feeding strategy that maximises Pseudomonas sp. GO16 biomass synthesis. The obtained biomass concentrations of 10.5 g/L (liquid-phase feeding) and 15.3 g/L (solid-phase feeding) are the highest ever reported using Na2TA as a sole carbon source for microbial growth. |
| Databáze: | OpenAIRE |
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